thepresenceof monovalent human soluble recombinant pecam 1 srpecam 1  (R&D Systems)

Journal:

Article Title: Fresh Isolates from Children with Severe Plasmodium falciparum Malaria Bind to Multiple Receptors

doi: 10.1128/IAI.69.9.5849-5856.2001

Figure Lengend Snippet: Summary of data for each of the parameters studied and comparison between groups a

Article Snippet: Soluble(s) recombinant human PECAM-1/CD31 was purchased from R&D Systems (Minneapolis, Minn.) and subsequently labeled with the fluorophore Alexa 488 (Molecular Probes, Eugene, Oreg.) according to the instructions provided in the Alexa 488 labeling kit.

Techniques: Comparison, Binding Assay

Journal:

Article Title: Fresh Isolates from Children with Severe Plasmodium falciparum Malaria Bind to Multiple Receptors

doi: 10.1128/IAI.69.9.5849-5856.2001

Figure Lengend Snippet: Mosaic representing the frequency and magnitude of adhesion. Squares □, ░, ▒, ▓, and █ indicate adhesion magnitude and correspond to scores of 1 to 5, respectively, where 1 represents low or no binding and 5 indicates high binding. For different parameters, scores represent different percentages of pRBC involved, as follows. For rosetting, 1 = 0 to 9%; 2 = 9 to 18%; 3 = 18 to 27%; 4 = 27 to 36%; 5 = >36%. For giant rosetting, 1 = 0 to 4%; 2 = 4 to 8%; 3 = 8 to 12%; 4 = 12 to 16%; 5 = >16%. For immunoglobulin binding, 1 = 0 to 7%; 2 = 7 to 14%; 3 = 14 to 21%; 4 = 21 to 28%; 5 = >28%. For heparin and sCD31 binding, 1 = 0 to 6%; 2 = 6 to 12%; 3 = 12 to 18%; 4 = 18 to 24%; 5 = >24%. For the number of pRBC bound to CD31- and CD36-transfected L or CHO cells, 0 to 100 pRBC/100 transfectants; 2 = 100 to 200/100 transfectants; 3 = 200 to 300/100 transfectants; 4 = 300 to 400/100 transfectants; 5 = >400/100 transfectants. Asterisks indicate isolates with binding scores of >1 in more than four categories.

Article Snippet: Soluble(s) recombinant human PECAM-1/CD31 was purchased from R&D Systems (Minneapolis, Minn.) and subsequently labeled with the fluorophore Alexa 488 (Molecular Probes, Eugene, Oreg.) according to the instructions provided in the Alexa 488 labeling kit.

Techniques: Binding Assay, Transfection

Journal: The Journal of Experimental Medicine

Article Title: The Semiconserved Head Structure of Plasmodium falciparum Erythrocyte Membrane Protein 1 Mediates Binding to Multiple Independent Host Receptors

doi:

Figure Lengend Snippet: Binding of Different Receptors and RBCs to var Fragment–transfected COS-7 Cells

Article Snippet: Soluble PECAM-1/CD31 purified from Chinese hamster ovary (CHO) cells (cat. no. ADP6) was purchased from R&D Systems.

Techniques: Binding Assay, Transfection